Assassin's Creed: Brotherhood, Leonardo da Vinci(/Salai), Among those to whom "nothing is true and everything is permitted", Leonardo finds a freedom to speak about the one thing he's forced himself to keep quiet about.

Assassin's Creed: Brotherhood, Leonardo da Vinci, what happened after his accidental coming out in the Temple of Pythagoras.

Bioshock, any character, Rapture's attitude towards queer people is different than that of the surface above--especially when splicing makes your body far easier to reshape. (Bonus points for incorporation of the film advertised on the left)

Bioshock Infinite, the Lutece Twins, their constants and their variables.

Welcome to Night Vale, Cecil Palmer/Carlos, Carlos takes Cecil on a trip outside of Night Vale, 'where things are less confusing and scary'. He explains about harmless street cleaners, the lack of government surveillance, and the acceptability of wheat by-products...he forgets to explain why Cecil can't kiss him in public. Cecil does not understand why this is less confusing and scary.

Any fandom, any ensemble cast, "Wait, we're all queer?" Finding out that you're not the minority, but the majority.

Any fandom, any character, using your secret identity as a way to display your true gender.

Any fandom, any character, the one person you thought would reject you turns out to be the one that's the most supportive.